Continuous fluidized bed chromatography for bioseparations

Dr. Amarjeet Bassi, Professor of Chemical and Biochemical Engineering, Associate Dean (Academic), Faculty of Engineering, University of Western Ontario

26 February 2009 at 10:30

Location: JHE 326H

Downstream processing is an essential component of any biotechnology or pharmaceutical process. Traditionally, packed bed or expanded fluidized bed chromatography is applied as one of the steps in such operations. However such an approach is cyclic and cannot achieve simultaneous adsorption and desorption in the same unit. In order to fully realize the advantages of the fluidized bed, we have developed a new continuous multi-column fluidized bed ion exchanger which operates as a single unit. The adsorption and desorption (regeneration) are carried out separately in two separate and inter- connected columns in a continuous mode with the ion exchange particles circulating between them. A unique feature of this system is that un-clarified whole broth with small particulates and other contaminants can be treated directly with no pre-clarification necessary for the feed, therefore eliminating an entire step in the downstream processing train. Some applications including protein recovery, organic acids separation and biopharmaceutical recovery from whole fermentation broth will be discussed.


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