Controlling Protein Adsorption on Biomaterial Surfaces: Prevent Nonspecific and Encourage Specific Interactions

Xudong Deng, PhD Candidate, Department of Chemical Engineering, McMaster University


28 March 2016 at 12:30

Location: IAHS B102

Biomaterials, synthetic or natural, are materials that used in contact with biological systems in diagnostic and medical devices. When a material comes into contact with a biological fluid system containing proteins, blood, cells or tissues, a cascade of interdependent events take place and generate signals, which determines the destiny of subsequent bio/material interactions. On one hand, nonspecific protein binding on the surfaces always occurs rapidly, and the adsorbed protein quickly forms a fibrous avascular capsule, which isolates the device from its target environment, hinders the effectiveness of the interfacial function, or decrease the sensitivity of biosensors. Therefore, the prevention of nonspecific protein adsorption plays a key role in improving the biocompatibility of biomaterials. On the other hand, immobilization of bioactive proteins on bioinert surfaces enables both localization and retention of the required functions at the bio/material interface. Herein, a interfacial coating layer composed of a synthetic polymer, poly(oligoethylene glycol methacrylate) (POEGMA) or a natural polymer, hyaluronic acid (HA) have been employed to effectively prevent the nonspecific protein adsorption on surfaces, where cellulose paper and poly(2-hydroxyethyl methacrylate) (PHEMA) serve as the basic biomaterials respectively. In addition, biotinlated graphene oxide is introduced here as a universal bifunctional anchoring molecule that enables antibody immobilization on a quartz crystal microbalance (QCM) gold sensor surface, which could largely improve the sensitivity of the antigen detection.


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