Department of Chemical Engineering
Canada Research Chair in Bioseparations
1280 Main Street West, Hamilton
Ontario, Canada L8S 4L7
voice: (905) 525-9140 ext. 27415
fax: (905) 521-1350
D.Phil.Chemical Engineering, University of Oxford (1999)
Bioseparation using ultrafiltration
Development of efficient, scalable, high-resolution purification processes
is essential for cost-effective bio-pharmaceutical manufacturing. We are
working on techniques for rapid optimization of ultrafiltration processes
and on the development of novel and efficient cascade ultrafiltration
systems for purification of serum and monoclonal antibodies. Other interests
include development of smart environment responsive membranes and study
of membrane fouling.
Bioseparation using membrane chromatography
Our interests include the development of economic and efficient paper
based adsorptive membranes and the design of novel membrane adsorption
devices. Typical applications we are currently working on include monoclonal
antibody purification, antibody aggregate separation, purification of
therapeutic proteins from transgenic tobacco and plasma protein purification.
We have developed of a novel hybrid bioseparation technique based on the
combination of protein precipitation, sieving and hydrophobic interaction
membrane adsorption for high resolution separation of antibodies. Typical
applications include monoclonal antibody purification, hyper-immune antibody
production and plasma fractionation.
Membrane based analytical bioseparations
We developed a novel and efficient method for detecting and analyzing
antibody aggregates based on the use of hydrophobic interaction membranes.
We are also working on efficient way of using membranes for immunoassays
– in a membrane chromatographic mode. This approach has opened up
some new opportunities such as rapid multi-target testing which are being
Engineering Research Group
R.Ghosh, Protein Bioseparation Using Ultrafiltration:
Theory, Applications and New Developments, Imperial College Press/World
Scientific Publishing Pte Ltd. ISBN 1-86094-317-9 (June 2003)
R. Ghosh, Principles of Bioseparations Engineering, World Scientific
Publishing Pte Ltd. ISBN 981-256-892-1 (October 2006).
Recent Refereed journal papers
* corresponding author
1. D.Q. Yu, M.D. McLean, J.C Hall, R. Ghosh*, Purification of a human immunoglobulin G1 monoclonal antibody from transgenic tobacco using membrane chromatographic processes, Journal of Chromatography A, 1187 (2008) 128.
2. L. Wang, R. Ghosh*, Fractionation of monoclonal antibody aggregates using membrane chromatography, Journal of membrane Science, 318 (2008) 311.
3. D. Q. Yu, M.D. McLean, J.C. Hall, R. Ghosh*, Purification of monoclonal antibody from tobacco extract using membrane-based bioseparation techniques, Journal of Membrane Science (in press, 2008).
4. D. M. Kanani, X. Sun, R. Ghosh*, Reversible and irreversible membrane fouling during in-line microfiltration of concentrated protein solutions, Journal of Membrane Science, 315 (2008) 1.
5. X. Sun, D.M. Kanani, R. Ghosh*, Characterization and theoretical analysis of protein fouling of cellulose acetate membrane during constant flux dead-end microfiltration, Journal of Membrane Science, 320 (2008) 372.
6. K. Mohanty, R. Ghosh*, Novel tangential-flow countercurrent cascade ultrafiltration configuration for continuous purification of humanized monoclonal antibody, Journal of Membrane Science, 1307 (2008) 117.
7. L. K. Kostanski*, R. X. Huang, R. Ghosh, C.D.M. Filipe, Biocompatible poly(N-vinyllactam)-based materials with environmentally-responsive permeability, Journal of Biomaterials Science-Polymer Edition, 19 (2008) 275.
8. D. Yu, X. Chen, R. Pelton, R. Ghosh*, Paper-PEG-based membranes for hydrophobic interaction chromatography: Purification of monoclonal antibody, Biotechnology and Bioengineering, 99 (2008) 1434.
9. L. Wang, X. Sun, R. Ghosh*, Purification of equine IgG using membrane based enhanced hybrid bioseparation technique: A potential method for manufacturing hyperimmune antibody, Biotechnology and Bioengineering, 99 (2008) 625.
10. D. M. Kanani, R. Ghosh*, A constant flux based mathematical model for predicting permeate flux decline in constant pressure protein ultrafiltration, Journal of Membrane Science 290 (2007) 207.
11. D. R. Latulippe, R. Ghosh, C.D.M. Filipe*, Pulse injection technique for membrane characterization: Ultrafiltration of dextran solutions, Journal of Membrane Science 289 (2007) 268.
12. D. M. Kanani, E. Komkova, T. Wong, A. Mika, R.F. Childs, R. Ghosh*, Separation of human plasma proteins HSA and HIgG using high-capacity macroporous gel-filled membranes, Biochemical Engineering Journal 35 (2007) 295.
13. R. Ghosh*, T. Wong, Effect of module design on the efficiency of membrane chromatographic separation processes, Journal of Membrane Science, 281 (2006) 532.
14. L. Wang, D.M. Kanani, R. Ghosh*, Purification of humanized monoclonal antibodies by membrane-based hybrid bioseparation technique, Journal of Immunological Methods, 314 (2006) 1.
15. L. Wang, G. Hale, R. Ghosh*, Non-size-based membrane chromatographic separation and analysis of monoclonal antibody aggregates, Analytical Chemistry, 78 (2006) 6863.
16. R. Ghosh*, Rapid antibody screening by membrane chromatographic immunoassay technique, Journal of Chromatography B, 844 (2006) 163.
17. R. Ghosh*, Enhancement of membrane permeability by gas-sparging in submerged hollow fibre ultrafiltration of macromolecular solutions: Role of module design, Journal of Membrane Science, 274 (2006) 73.
18. R. Ghosh*, L. Wang, Purification of humanized monoclonal antibody by hydrophobic interaction membrane chromatography, Journal of Chromatography A, 1107 (2006) 104.
19. R. Ghosh*, Membrane chromatographic immunoassay method for rapid quantitative analysis of specific serum antibodies, Biotechnology and Bioengineering, 93 (2006) 280.