Dr. David Latulippe
David Latulippe
Assistant Professor
Department of Chemical Engineering
1280 Main Street West,
Hamilton, ON, L8S 4L7, Canada
Driving directions and map

Office: JHE 345B
Voice: +1 (905) 525 9140 extension 24011
email: latulippe@mcmaster.ca

separation processes for water/wastewater treatment, biopartitioning and purification, microscale processing for high-throughput studies,unit operation modeling and optimization
Education
  • B. Eng. Chemical Engineering, McMaster University (2000)
  • M.A.Sc. Chemical Engineering, McMaster University (2005)
  • Ph.D. Chemical Engineering, Pennsylvania State University (2010)
  • Post-Doctoral Associate Applied & Engineering Physics, Cornell University (2010-2012)

Research Interests

The overarching theme of my research group is the development and optimization of novel separation processes.  Toward this end, we typically focus on microscale processes (MSP) - the study of conventional processes (e.g. chromatography, membrane filtration, adsorption) but in miniaturized formats such as micro-columns, micro-well plates, and microfluidic devices. The advantages of this approach are considerable - it is extremely cost-effective since it requires minimal amounts of material and it is very efficient since it uses a parallel architecture approach. Our research uses a unique combination of experimental work, statistical analysis, and advanced tools for sample characterization and process modelling.

Microscale processing in environmental engineering:

We are developing novel separation processes for a variety of 'tough-to-treat' applications including tailings (i.e. suspensions of clay particles) from mining operations, biosolids from municipal WW treatment, and vegetable 'wash-water' from agricultural processing.  Our work includes both physical, chemical and biological-based processes including the development of the first ever high-throughput microscale stirred cell filtration device for flat sheet membranes, the first ever high-throughput microscale filtration device for hollow fiber membranes, a lab-scale anaerobic digester featuring recuperative thickening technology.  Our lab is home to the Micro-scale Separations and Analysis Suite (MSAS), a collection of specialized equipment that is essential for the execution and analysis of micro-scale experiments, that was supported by the Canada Foundation for Innovation (CFI), Ontario Research Fund (ORF), and NSERC Research Tools and Instrumentation (RTI) programs.

Downstream purification of oncolytic viruses:

We are working to solve the considerable challenges associated with the downstream purification of oncolytic viruses (OVs), an innovative anti-cancer therapeutic that has attracted considerable attention. The establishment of a cost-effective OV manufacturing process is critically dependent on the development of new technologies for downstream processing - the finished product must be of a sufficiently high purity and immunogenic activity (to fulfill regulatory demands) that is ready for clinical trials and full-scale commercialization.  This work is being done in collaboration with scientists at the Ottawa Hospital Research Institute and University of Toronto through the Alliance for Biotherapeutics Manufacturing Innovation (ABMI).

 


Project Title


Industrial Partner

Supporting Funding Agency

Industrial wastewater treatment within the "design space" scientific framework

 


http://www.aevitas.ca

 

OCE

 

 Development of next-generation engineered textiles for sludge and tailings dewatering applications

 

 


http://cgtower.com

 

OCE

 


 

 

Quantitative evaluation of biocide contamination in industrial wastewater using PeCOD technology


http://www.mantech-inc.com

NSERC

 

Positions Available:

  • Undergraduate Students – Undergraduate research is an outstanding learning experience both for students seeking careers in industry and those interested in graduate school. NSERC has funding available specifically for undergraduate students – for more details see http://www.nserc-crsng.gc.ca/students-etudiants/ug-pc/usra-brpc_eng.asp. Senior-year students in any of the chemical engineering programs have the opportunity to join our lab as part of the ChE 4Y4 independent project course. Interested students should email
    Dr. David Latulippe (preferably with an attached resume).
  • Graduate students – There are positions available in our group for outstanding, highly-motivated and enthusiastic students. Those interested in joining the group should follow the application instructions on thedepartment website Inquiries about our research by prospective graduate students are strongly encouraged – please email Dr. David Latulippe. However, administrative questions relating to the admissions process should be directed to the graduate secretary

 

Selected Publications:

A.S. Kazemi, L. Boivin, S.M. Yoo, R. Ghosh, D.R. Latulippe, "Elucidation of filtration performance of hollow-fiber membranes via a high-throughput screening platform", Journal of Membrane Science
http://dx.doi.org/10.1016/j.memsci.2017.03.042

J. Cobbledick, V. Zhang, S. Rollings-Scattergood, D.R. Latulippe, "Investigation of the role of flocculation conditions in recuperative thickening on dewatering performance and biogas production", Environmental Technology
http://dx.doi.org/10.1080/09593330.2016.1272639

A.S. Kazemi, K. Kawka, D.R. Latulippe, "Optimization of bio-molecule separation by combining microscale filtration and design-of-experiment methods", Biotechnology & Bioengineering
http://onlinelibrary.wiley.com/doi/10.1002/bit.25975/full

J. Cobbledick, N. Aubry, V. Zhang, S. Rollings-Scattergood, D.R. Latulippe, "Lab-scale demonstration of recuperative thickening technology for enhanced biogas production and dewaterability in anaerobic digestion processes", Water Research 95 (2016) 39-47
http://www.sciencedirect.com/science/article/pii/S0043135416301105

R.J. LaRue, J. Cobbledick, N. Aubry, E.D. Cranston, D.R. Latulippe, "The microscale flocculation test (MFT) - a high-throughput technique for optimizing separation performance", Chemical Engineering Research and Design 105 (2016) 85–93
http://www.sciencedirect.com/science/article/pii/S0263876215004190

A. S. Kazemi, D.R. Latulippe, "Stirred well filtration (SWF) - a high-throughput technique for downstream bio-processing" Journal of Membrane Science 470 (2014) 30-39.
http://www.sciencedirect.com/science/article/pii/S0376738814005377

J. Cobbledick, A. Nguyen, D.R. Latulippe, "Demonstration of FBRM as process analytical technology tool for dewatering processes via CST correlation" Water Research 58 (2014) 132-140.
 

 

K. Szeto, D. R. Latulippe, A. Ozer, J.M. Pagano, B. S. White, D. Shalloway, J.T. Lis, H.G. Craighead. RAPID-SELEX for RNA aptamers", PLOS ONE 8 (2013) e82667

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0082667

  

D.R. Latulippe, K. Szeto, A. Ozer, F.M. Duarte, C.V. Kelly, J.M. Pagano, B.S. White, D. Shalloway, J.T. Lis, H.G. Craighead, “Multiplexed microcolumn-based process for efficient selection of RNA aptamers”, Analytical Chemistry 85 (2013) 3417-3424
http://pubs.acs.org/doi/abs/10.1021/ac400105e 

 
J.J. Benitez, J. Topolancik, H.C. Tian, C.B. Wallin, D.R. Latulippe, K. Szeto, P.J. Murphy, B.R. Cipriany, S.L. Levy, P.D. Soloway, H.G. Craighead, "Microfluidic extraction, stretching and analysis of human chromosomal DNA from single cells”, Lab Chip 12 (2012) 4848-4854.
http://pubs.rsc.org/en/content/articlelanding/2012/lc/c2lc40955k


B. R. Cipriany, P. J. Murphy, J. A. Hagarman, A. Cerf, D.R. Latulippe, S. L. Levy, J. J. Benitez, C. P. Tan, J. Topolancik, P. D. Soloway, H. G. Craighead, "Real-time analysis and selection of methylated DNA by fluorescence-activated single molecule sorting in a nanofluidic channel", Proc Natl Acad Sci USA 109 (2012) 8477-8482.
http://www.pnas.org/content/early/2012/05/07/1117549109.abstract

D.R. Latulippe, A.L. Zydney, “Separation of plasmid DNA isoforms by highly converging flow through small membrane pores”, J Colloid Int Sci 357 (2011) 548-553
http://www.sciencedirect.com/science/article/pii/S0021979711001895

D.R. Latulippe, A.L. Zydney, “Radius of gyration of plasmid DNA isoforms from static light scattering”, Biotech Bioeng 107 (2010) 134-142
http://onlinelibrary.wiley.com/doi/10.1002/bit.22787/abstract

D.R. Latulippe, A.L. Zydney, “Size Exclusion Chromatography of Plasmid DNA Isoforms”, J Chromatogr A 1216 (2009) 6295-6302
http://www.sciencedirect.com/science/article/pii/S0021967309010450

D.R, Latulippe, K. Ager, A.L. Zydney, “Flux-dependent transmission of supercoiled plasmid DNA through ultrafiltration membranes”, J Membrane Sci 294 (2007) 169-177
http://www.sciencedirect.com/science/article/pii/S0376738807001330

 

Patents and Patent Applications:

H. G. Craighead, D.R. Latulippe, J.T. Lis, A. Ozer, K. Szeto, “RNA Aptamer Isolation via Dual-Cycle (RAPID) Selection”. U.S. Provisional Appl. Serial No. 61/683,381

H. G. Craighead, D.R. Latulippe, J.T. Lis, A. Ozer, K. Szeto, “Multiplexed micro-column devices for selection of nucleic acid aptamers”. U.S. Provisional Appl. Serial No. 61/645,783

D.R. Latulippe, A.L. Zydney, “Separation of different isoforms of plasmid DNA using ultrafiltration”. U.S. Patent No. 7,989,615 B2 (Aug. 2, 2011).