Dr. David Latulippe
David Latulippe
Assistant Professor
Department of Chemical Engineering
1280 Main Street West,
Hamilton, ON, L8S 4L7, Canada
Driving directions and map

Office: JHE 345B
Voice: +1 (905) 525 9140 extension 24011
email: latulippe@mcmaster.ca

separation processes for water/wastewater treatment, biopartitioning and purification, microscale processing for high-throughput studies,unit operation modeling and optimization
  • B. Eng. Chemical Engineering, McMaster University (2000)
  • M.A.Sc. Chemical Engineering, McMaster University (2005)
  • Ph.D. Chemical Engineering, Pennsylvania State University (2010)
  • Post-Doctoral Associate Applied & Engineering Physics, Cornell University (2010-2012)

Research Interests

My current research activities are focused on separation processes for biopolymer processing and wastewater treatment.

Toward this end, we typically focus on microscale processes (MSP) - the study of conventional processes (e.g. chromatography, membrane filtration, adsorption) but in miniaturized formats such as micro-columns, micro-well plates, and microfluidic devices. The advantages of this approach are considerable. First, it is extremely cost-effective since it requires minimal amounts of material. Thus, it can be used to thoroughly explore the entire process design space of a process and determine the optimum operating conditions. Second, it is very efficient since it uses a parallel architecture approach. Thus, this format allows for a high-throughput development strategy instead of the more common hypothesis driven approach. Finally, integrated instrumentation can be used to monitor in real-time the process performance.

The unifying theme of our work is to develop the appropriate structure-processing relationships and mechanistic models that predict the process performance. We frequently use various bio-physical tools (e.g., light scattering, electrophoresis, circular dichroism) and microscopic techniques (e.g., scanning electron microscopy). Our work is greatly enhanced by the resources that are available at the Biointerfaces Institute and the Brockhouse Institute for Materials Research. The projects in my group are multi-disciplinary in nature and typically include collaborations with polymer physicists, micro-biologists, and chemists.

Biopolymers are a diverse and versatile class of materials including polynucleotides, polypeptides, polysaccharides, and polyesters. We are particularly interested in developing new separation processes that solve the technical and economical challenges associated biopolymer production at various scales.

Our interests in wastewater lie at the water-energy nexus. This includes the treatment of water from hydraulic fracturing operations for natural gas production, as well as water demands in renewable energy (e.g. bio-gas) production. A list of current (as of September 2016) research projects related to wastewater treatment are given in the table below.

Project Title

Industrial Partner

Supporting Funding Agency

Industrial wastewater treatment within the "design space" scientific framework






 Development of next-generation egineered textiles for sludge and tailings dewatering applications






Industrial wastewater treatment within the "design space" scientific framework



Quantitative evaluation of biocide contamination in industrial wastewater using PeCOD technology




Positions Available:

  • Undergraduate Students – Undergraduate research is an outstanding learning experience both for students seeking careers in industry and those interested in graduate school. NSERC has funding available specifically for undergraduate students – for more details see http://www.nserc-crsng.gc.ca/students-etudiants/ug-pc/usra-brpc_eng.asp. Senior-year students in any of the chemical engineering programs have the opportunity to join our lab as part of the ChE 4Y4 independent project course. Interested students should email
    Dr. David Latulippe (preferably with an attached resume).
  • Graduate students – There are positions available in our group for outstanding, highly-motivated and enthusiastic students. Those interested in joining the group should follow the application instructions on thedepartment website Inquiries about our research by prospective graduate students are strongly encouraged – please email Dr. David Latulippe. However, administrative questions relating to the admissions process should be directed to the graduate secretary


Selected Publications:

A.S. Kazemi, K. Kawka, D.R. Latulippe, "Optimization of bio-molecule separation by combining microscale filtration and design-of-experiment methods", Biotechnology & Bioengineering

J. Cobbledick, N. Aubry, V. Zhang, S. Rollings-Scattergood, D.R. Latulippe, "Lab-scale demonstration of recuperative thickening technology for enhanced biogas production and dewaterability in anaerobic digestion processes", Water Research 95 (2016) 39-47

R.J. LaRue, J. Cobbledick, N. Aubry, E.D. Cranston, D.R. Latulippe, "The microscale flocculation test (MFT) - a high-throughput technique for optimizing separation performance", Chemical Engineering Research and Design 105 (2016) 85–93

A. S. Kazemi, D.R. Latulippe, "Stirred well filtration (SWF) - a high-throughput technique for downstream bio-processing" Journal of Membrane Science 470 (2014) 30-39.

J. Cobbledick, A. Nguyen, D.R. Latulippe, "Demonstration of FBRM as process analytical technology tool for dewatering processes via CST correlation" Water Research 58 (2014) 132-140.
K. Szeto, D. R. Latulippe, A. Ozer, J.M. Pagano, B. S. White, D. Shalloway, J.T. Lis, H.G. Craighead. RAPID-SELEX for RNA aptamers", PLOS ONE 8 (2013) e82667
D.R. Latulippe, K. Szeto, A. Ozer, F.M. Duarte, C.V. Kelly, J.M. Pagano, B.S. White, D. Shalloway, J.T. Lis, H.G. Craighead, “Multiplexed microcolumn-based process for efficient selection of RNA aptamers”, Analytical Chemistry 85 (2013) 3417-3424
J.J. Benitez, J. Topolancik, H.C. Tian, C.B. Wallin, D.R. Latulippe, K. Szeto, P.J. Murphy, B.R. Cipriany, S.L. Levy, P.D. Soloway, H.G. Craighead, "Microfluidic extraction, stretching and analysis of human chromosomal DNA from single cells”, Lab Chip 12 (2012) 4848-4854.

B. R. Cipriany, P. J. Murphy, J. A. Hagarman, A. Cerf, D.R. Latulippe, S. L. Levy, J. J. Benitez, C. P. Tan, J. Topolancik, P. D. Soloway, H. G. Craighead, "Real-time analysis and selection of methylated DNA by fluorescence-activated single molecule sorting in a nanofluidic channel", Proc Natl Acad Sci USA 109 (2012) 8477-8482.

D.R. Latulippe, A.L. Zydney, “Separation of plasmid DNA isoforms by highly converging flow through small membrane pores”, J Colloid Int Sci 357 (2011) 548-553

D.R. Latulippe, A.L. Zydney, “Radius of gyration of plasmid DNA isoforms from static light scattering”, Biotech Bioeng 107 (2010) 134-142

D.R. Latulippe, A.L. Zydney, “Size Exclusion Chromatography of Plasmid DNA Isoforms”, J Chromatogr A 1216 (2009) 6295-6302

D.R, Latulippe, K. Ager, A.L. Zydney, “Flux-dependent transmission of supercoiled plasmid DNA through ultrafiltration membranes”, J Membrane Sci 294 (2007) 169-177


Patents and Patent Applications:

H. G. Craighead, D.R. Latulippe, J.T. Lis, A. Ozer, K. Szeto, “RNA Aptamer Isolation via Dual-Cycle (RAPID) Selection”. U.S. Provisional Appl. Serial No. 61/683,381

H. G. Craighead, D.R. Latulippe, J.T. Lis, A. Ozer, K. Szeto, “Multiplexed micro-column devices for selection of nucleic acid aptamers”. U.S. Provisional Appl. Serial No. 61/645,783

D.R. Latulippe, A.L. Zydney, “Separation of different isoforms of plasmid DNA using ultrafiltration”. U.S. Patent No. 7,989,615 B2 (Aug. 2, 2011).